*Co-first authors #Corresponding author(s) Shikuma Lab Members
AT Alker, MV Farrell, AM Demko, TN Purdy, S Adak, BS Moore, JM Sneed, VJ Paul, NJ Shikuma# (2023) Linking bacterial tetrabromopyrrole to coral metamorphosis. ISME Communications. https://doi.org/10.1038/s43705-023-00309-6
AT Alker, A Aspiras, TL Dunbar, MV Farrell, A Fedoriouk, J Jones, S Michael, GY Salcedo, BS Moore, NJ Shikuma# (2023) A Modular Plasmid Toolkit Applied in Marine Bacteria Reveals Functional Insights During Bacteria-Stimulated Metamorphosis. mBio. https://doi.org/10.1128/mbio.01502-2
KT Nesbit and NJ Shikuma# (2023). Developmental staging of the complete life cycle of the model marine tubeworm Hydroides elegans. Developmental Dynamics.https://doi.org/10.1002/dvdy.628
KE Malter, M Esmerode, AT Alker, M Damba, EM Forsberg and NJ Shikuma# (2022). Diacylglycerol, PKC and MAPK signaling initiate tubeworm metamorphosis in response to bacteria. Developmental Biology. 487:99-109 DOI: 10.1016/j.ydbio.2022.04.009
AT Alker, NA Hern, MA Ali, MI Baez, BC Baswell, BI Baxter, A Blitz, TM Calimlim, CA Chevalier, CA Eguia, T Esparza, AE Fuller, CJ Gwynn, AL Hedin, RA Johnson, M Kaur, RT Laxina, K Lee, PN Maguire, IF Martelino, JA Melendez, J J Navarro, JN Navarro, JM Osborn, MR Padilla, ND Peralta, JLR Pureza, JJ Rojas, TR Romo, M Sakha, GJ Salcedo, KA Sims, TH Trieu, IR Niesman, NJ Shikuma# (2022). Draft Genome Sequence of Nereida sp. Strain MMG025 Isolated from Giant Kelp. Microbiology Resource Announcements. https://doi.org/10.1128/mra.00122-22
NJ Shikuma# (2021). Bacteria-Stimulated Metamorphosis: An Ocean of Insights from Investigating a Transient Host-Microbe Interaction. mSystems. https://doi.org/10.1128/mSystems.00754-21. Invited Early Career Special Collection.
An invited perspective piece describing our vision for the field of bacteria-stimulated metamorphosis and beyond.
AT Alker, B Gode, A Aspiras, J Jones, S Michael, D Aguilar, A Cain, AM Candib, J Cizmic, E Clark, AC Cozzo, L Figueroa, P Garcia, C Heaney, AT Levy, L Macknight, A McCarthy, JP McNamara, K Nguyen, KN Rollin, GY Salcedo, JA Showalter, A Sue, TR Zamro, TL Dunbar, KE Malter, and NJ Shikuma# (2021). Draft Genome Sequences of Ten Bacteria from the Marine Pseudoalteromonas Group. Microbiology Resource Announcements. https://doi.org/10.1128/MRA.00404-21
This is an NSF-funded publication promoting undergraduate engagement in discovery-based research.
23 undergraduate students are substantial contributors and co-authors of this work.
AT Alker, N Delherbe, T Purdy, B Moore, NJ Shikuma# (2020). Genetic examination of the marine bacterium Pseudoalteromonas luteoviolacea and effects of its metamorphosis-inducing factors. Environmental Microbiology. https://doi.org/10.1111/1462-2920.15211
We uncover a hidden complexity of interactions between a single bacterial species, the factors it produces and two species of animal larvae.
GS Cavalcanti, AT Alker, N Delherbe, KE Malter, NJ Shikuma# (2020). The Influence of Bacteria on Animal Metamorphosis. Annual Review of Microbiology. 74:137-58. https://doi.org/10.1146/annurev-micro-011320-012753
We review what is known and what we hope to learn about how bacteria and the factors they produce stimulate animal metamorphosis.
Maria I. Rojas*, Giselle S. Cavalcanti*, Katelyn McNair, Sean Benler, Amanda T. Alker, Ana G. Cobián-Güemes, Melissa Giluso, Kyle Levi, Forest Rohwer, Barbara Bailey, Sinem Beyhan, Robert A. Edwards, Nicholas J. Shikuma# (2020). A Distinct Contractile Injection System Gene Cluster Found in a Majority of Healthy Adult Human Microbiomes. mSystems 5:e00648-20. https://doi.org/10.1128/MSYSTEMS.00648-20
We discovered a yet undescribed Injection System genecluster in the microbiomes of nearly all adult humans.
CEricson*, FEisenstein*, J Medeiros, K Malter, G Cavalcanti, RW Zeller, DK Newman, MPilhofer# andNJShikuma# (2019). A Contractile Injection System Stimulates Tubeworm Metamorphosis by Translocating a Proteinaceous Effector. eLife 8:e46845.https://doi.org/10.7554/eLife.46845.
I Rocchi*, C Ericson*, KE Malter*, S Zargar, F Eisenstein, M Pilhofer, S Beyhan# and NJ Shikuma# (2019). A Bacterial Phage Tail-like Structure Kills Eukaryotic Cells by Injecting a Nuclease Effector. Cell Reports 28(2), 295-301.https://doi.org/10.1016/j.celrep.2019.06.019.
NJ Shikuma, JCN Fong and FH Yildiz (2012) Cellular levels and binding of c-di-GMP control subcellular localization and activity of the Vibrio cholerae transcriptional regulator VpsT. PLoS Pathogens 8(5):e1002719.
PV Krasteva, JCN Fong, NJ Shikuma, S Beyhan, MVAS Navarro, FH Yildiz and H Sondermann (2010)Vibrio cholerae VpsT regulates matrix production and motility by directly sensing cyclic di-GMP. Science 327:866-868.
NJ Shikuma and FH Yildiz (2009) Identification and characterization of OscR, a transcriptional regulator involved in osmolarity adaptation in Vibrio cholerae. Journal of Bacteriology191:4082-4096.